CrossLaps peptide is a part of the C-telopeptide of the beta 1-chain of type I collagen of bone, is a recently developed biochemical marker of bone turnover.Serum beta-crosslaps are an established laboratory test to investigate bone metabolism. However, lytic conditions may affect the measurement of serum beta-crosslaps. the influence of proteolytic conditions on the serum concentration of beta-crosslaps is not uniform. Leukocyte lysate decreased serum beta-crosslaps while the addition of cathepsin C increased their concentration. Therefore, serum should be separated from the whole blood immediately after coagulation and stored until analysis in a deep freezer. Aliases: B-CTx; Beta CrossLaps; Beta-CTx; C-Telopeptide; C-terminal collagen crosslinks; Carboxy terminal collagen crosslinks; CTx; Soft-CTX

Human Beta-crosslaps (BCTX) ELISA Kit employs a two-site sandwich ELISA to quantitate BCTX in samples. An antibody specific for BCTX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBCTX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BCTX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BCTX bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Beta-crosslaps (BCTX) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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