BAT3 contains an N-terminal ubiquitin-like domain, and both BAT2 and BAT3 are rich in proline and include short tracts of polyproline, polyglycine, and charged amino acids.The presence of additional genes was suggested by a large cluster of CpG islands. With cosmid probes, 5 distinct transcripts, including BAT3, were detected in RNA samples from a variety of cell lines, and the corresponding cDNA clones were isolated.BAT3 formed a complex with p300 (EP300), and an increased amount of BAT3 enhanced recruitment of p53 to p300 and facilitated subsequent p53 acetylation. In contrast, Bat3-depleted cells showed reduced p53-p300 complex formation and decreased p53 acetylation. Thymocytes from Bat3-deficient mice exhibited reduced p53-mediated induction of Puma and p21 and were resistant to DNA damage-induced apoptosis in vivo.

Human HLA-B associated transcript 3 (BAT3) ELISA Kit employs a two-site sandwich ELISA to quantitate BAT3 in samples. An antibody specific for BAT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBAT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BAT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BAT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human HLA-B associated transcript 3 (BAT3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-hla-b-associated-transcript-3-bat3-elisa-kit-kte62697/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.