The C-terminus (also known as the carboxyl-terminus, carboxy-terminus, C-terminal tail, C-terminal end, or COOH-terminus) is the end of an amino acid chain (protein or polypeptide), terminated by a free carboxyl group (-COOH). When the protein is translated from messenger RNA, it is created from N-terminus to C-terminus. The convention for writing peptide sequences is to put the C-terminal end on the right and write the sequence from N- to C-terminus.

Human Autoantibodies against the C-terminal domain 1 (ACAST-D1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACAST-D1 in samples. An antibody specific for ACAST-D1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACAST-D1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACAST-D1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACAST-D1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Autoantibodies against the C-terminal domain 1 (ACAST-D1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.