ALCAM/CD166 is a member of the immunoglobulin gene superfamily belonging to a subgroup with five extracellular immunoglobulin-like domains(VVC2C2C2), which includes MCAM/CD146/MUC18 and B-CAM/Lutheran. These molecules are involved in the development and maintenance of tissue architecture, neurogenesis, hematopoiesis, immune responses and tumor progression. ALCAM/CD166 mediates cell-cell clustering through homophilic (ALCAM-ALCAM) and heterophilic (ALCAM-CD6) interactions. Its pattern of expression in human tissues and cells is broad and includes epithelia, neurons, lymphoid and myeloid cells, hematopoietic and mesenchymal stem cells. The relevance of the engagement of the heterophilic pair CD166-CD6 for optimal activation of T cells has been recently demonstrated.

Human Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit employs a two-site sandwich ELISA to quantitate ALCAM in samples. An antibody specific for ALCAM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyALCAM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALCAM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALCAM bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.