GPR109A is a high affinity receptor for nicotinic acid (niacin) and is a member of the nicotinic acid receptor family of G protein-coupled receptors (the other identified member being GPR109B).GPR109A is a Gi / Go protein-coupled receptor with high affinity for nicotinic acid.GPR109A is believed to be an important biomolecular target of niacin which is a widely prescribed drug for the treatment of dyslipidemia and to increase HDL cholesterol but whose therapeutic use is limited by flushing. In GPR109A knockout mice, the effects of niacin on both lipids and flushing is eliminated. Furthermore in arrestin beta 1 knockout mice, niacin's effect on flushing is greatly reduced while the lipid modifying effects are maintained.

Human G protein-coupled receptor 109A (GPR109A) ELISA Kit employs a two-site sandwich ELISA to quantitate GPR109A in samples. An antibody specific for GPR109A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGPR109A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPR109A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPR109A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human G protein-coupled receptor 109A (GPR109A) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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