CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. DNMT3A encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms.The mouse genes, termed Dnmt3a and Dnmt3b, show little sequence similarity to mouse Dnmt1 and Dnmt2, and masc1 from Ascobolus. The Dnmt3a cDNA encodes a protein of 908 amino acids. The human DNMT3A and DNMT3B cDNAs are highly homologous to the mouse genes. Dnmt3a and Dnmt3b transcripts were abundantly expressed in undifferentiated embryonic stem cells.

Human DNA (cytosine-5)-methyltransferase 3A (DNMT3A) ELISA Kit employs a two-site sandwich ELISA to quantitate DNMT3A in samples. An antibody specific for DNMT3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDNMT3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNMT3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNMT3A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human DNA (cytosine-5)-methyltransferase 3A (DNMT3A) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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