Aldosterone is a steroid hormone with a molecular weight of 360.4. It is secreted by the glomerulosa zone of the adrenal gland and controls regulation of hydromineral metabolism. In the distal part of the nephron, aldosterone encourages reabsorption of Na and Cl- ions and secretion of K and H ions in blood vessel lumen. Thus there is an increase in extra-cellular osmolarity and water retention. Regulation of aldosterone secretion is linked to the body's water balance and brings three factors into play. These are the renin- angiotensin system, the plasmic Na /K ratio and, as a secondary factor, the ACTH. Physiologically, there seems to be circadian rythm for levels of aldosterone in the blood, which may be linked to changes in body posture. Aldosterone levels are higher for children than for adults, and increase during pregnancy.

Human Aldolase (ALD) ELISA Kit employs a two-site sandwich ELISA to quantitate ALDOB in samples. An antibody specific for ALDOB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyALDOB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALDOB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALDOB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Aldolase (ALD) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.