Choline acetyltransferase (EC 2.3.1.7; abbreviated "ChAT") is an enzyme that is synthesized within the body of a neuron. It is then transferred to the nerve terminal via axoplasmic flow. The role of choline acetyltransferase is to join Acetyl-CoA to choliࣀ̐ࡰ̐

Human Choline acetyltransterase (ChAT) ELISA Kit employs a two-site sandwich ELISA to quantitate CHAT in samples. An antibody specific for CHAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCHAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHAT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHAT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Choline acetyltransterase (ChAT) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.