Members of the chemokine-like factor superfamily, including CKLFSF6, share similarity with both the chemokine and the transmembrane 4 superfamilies of signaling molecules.By screening databases for sequences that share homology with chemokine-like factor (CKLF), followed by RT-PCR of a prostate cDNA library, Han et al. (2003) cloned CKLFSF6. The deduced 183-amino acid CKLFSF6 protein has 4 transmembrane segments. It shares about 31% sequence identity with CKLFSF4 . Northern blot analysis detected a transcript of 3.88 kb expressed in leukocytes, placenta, and testis. A smaller transcript was also detected in leukocytes. Han et al. (2003) cloned mouse Cklfsf6, which shares about 88.5% sequence similarity with CKLFSF6.

Human CKLF-like MARVEL transmembrane domain-containing protein 6 (CMTM6) ELISA Kit employs a two-site sandwich ELISA to quantitate CMTM6 in samples. An antibody specific for CMTM6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCMTM6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CMTM6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CMTM6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human CKLF-like MARVEL transmembrane domain-containing protein 6 (CMTM6) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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