METTL5 belongs to the methyltransferase superfamily. PrmA family. A methyltransferase is a type of transferase enzyme that transfers a methyl group from a donor to an acceptor.Methylation often occurs on nucleic bases in DNA or amino acids in protein structures. Methytransferases use a reactive methyl group bound to sulfur in S-adenosyl methionine (SAM) as the methyl donor. Site-specific methyltransferases have the same DNA target sequences as certain restriction enzymes. Methylation can also serve to protect DNA from enzymatic cleavage, since restriction enzymes are unable to bind and recognize externally modified sequences. This is especially useful in bacterial restriction modification systems that use restriction enzymes to cleave foreign DNA while keeping their own DNA protected by methylation.

Human Methyltransferase-like protein 5 (METTL5) ELISA Kit employs a two-site sandwich ELISA to quantitate METTL5 in samples. An antibody specific for METTL5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMETTL5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for METTL5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of METTL5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Methyltransferase-like protein 5 (METTL5) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-methyltransferase-like-protein-5-mettl5-elisa-kit-kte62370/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.