POP5. The deduced 163-amino acid protein has a calculated molecular mass of 18.8 kD. Western blot analysis of HeLa cells detected endogenous POP5 at an apparent molecular mass of 19 kD.HeLa cell POP5 associated with RNase MRP and RNase P RNAs. The immunoprecipitated RNase P complex was able to cleave pre-tRNA into mature tRNA and its 5-prime leader sequence, indicating that POP5 is part of the catalytically active RNase P complex. Mutation analysis showed that the C terminus of POP5 was not required for association with RNase MRP or RNase P complexes or for RNase P activity.

Human Ribonuclease P/MRP protein subunit POP5 (POP5) ELISA Kit employs a two-site sandwich ELISA to quantitate POP5 in samples. An antibody specific for POP5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOP5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POP5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POP5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Ribonuclease P/MRP protein subunit POP5 (POP5) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.