The C114 cDNA contains an open reading frame of 187 amino acids with a predicted mass of 21 kDa. Using green fluorescent protein (GFP)-C114 fusion plasmids, amino acids 126-131 are shown to be essential for the nuclear localization of C114. An arginine-rich region (amino acids 98-143) spanning the nuclear localization signals (amino acids 126-131) exhibits a double-stranded RNA (dsRNA) binding activity. Competition experiments with different RNA homopolymers demonstrate that C114 preferentially binds to poly(I.C). C114 binds to the dsRNA-activated protein kinase, protein kinase R (PKR), via dsRNA-binding domains of PKR and the N-terminal region of the C114 protein. In vitro kinase assays indicate that C114 inhibits PKR activation via a dsRNA-independent mechanism.

Human PRKR-interacting protein 1 (PRKRIP1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKRIP1 in samples. An antibody specific for PRKRIP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKRIP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKRIP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKRIP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human PRKR-interacting protein 1 (PRKRIP1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.