RalGPS1 is a Ral GEF that requires membrane targeting to function in vivo.RalGPS 1 is a highly specific Ral GEF. Guanine nucleotide exchange factors (GEFs) are responsible for coupling cell surface receptors to Ras protein activation. These molecules share the core catalytic domain of other Ras family GEFs but lack the catalytic non-conserved domain that is believed to contribute to Sos1 integrity. In vitro binding and in vivo nucleotide exchange assays indicate that these GEFs specifically catalyze the GTP loading of the Ral GTPase when overexpressed in 293T cells. A central proline-rich motif associated with the Src homology (SH)2/SH3-containing adapter proteins Grb2 and Nck in vivo, whereas a pleckstrin homology (PH) domain was located at the GEF C terminus.

Human Ras-specific guanine nucleotide-releasing factor RalGPS1 (RALGPS1) ELISA Kit employs a two-site sandwich ELISA to quantitate RALGPS1 in samples. An antibody specific for RALGPS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRALGPS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RALGPS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RALGPS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Ras-specific guanine nucleotide-releasing factor RalGPS1 (RALGPS1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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