The 616-amino acid human semaphorin E contains an N-terminal signal sequence of 15 amino acids that is followed by a semaphorin domain. In vitro translation of the semaphorin E cDNA yielded a 70-kD protein. The authors showed that semaphorin E is secreted by mammalian cells. By Northern blot analysis, semaphorin E was expressed as a 5.2-kb transcript in all human tissues tested.CDDP-sensitive cells transfected with semaphorin E cDNA acquired CDDP-resistance. Semaphorin E was overexpressed in CDDP-resistant cell lines and was induced by diverse chemotherapeutic drugs and by x-ray and UV irradiation. Aberrant semaphorin E protein expression was detected immunohistochemically

Human Semaphorin-3C (SEMA3C) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA3C in samples. An antibody specific for SEMA3C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA3C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA3C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA3C bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Semaphorin-3C (SEMA3C) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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