CD14 exists in two forms. It is either anchored into the membrane by a glycosylphosphatidylinositol tail (mCD14) or it appears in a soluble form (sCD14). Soluble CD14 appears either after shedding of mCD14 (48 KDa) or is directly secreted from intracellular vesicles (56 KDa).CD14 takes its name from its inclusion in the cluster of differentiation group of cell surface marker proteins. CD14 was the first described pattern recognition receptor.CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can only bind LPS in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand CD14 also recognizes other pathogen associated molecular patterns.

Human Soluble cluster of differentiation 14 (sCD14) ELISA Kit employs a two-site sandwich ELISA to quantitate CD14 in samples. An antibody specific for CD14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCD14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD14 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Soluble cluster of differentiation 14 (sCD14) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.