kinase (EC 2.7.1.23, NADK) is an enzyme that converts nicotinamide adenine dinucleotide (NAD+) into NADP+, through phosphorylating the NAD+ coenzyme. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. The structure of the NADK from the archaean Archaeoglobus fulgidus has been determined.The deduced 446-amino acid protein has a calculated molecular mass of 49.2 kD. Northern blot analysis detected a 3.0-kb transcript with high expression in placenta, moderate expression in brain, colon, heart, liver, kidney, lung, spleen, stomach, and testis, and low expression in small intestine. A band of 1.8 kb was detected in several tissues.

Human NAD kinase (NADK) ELISA Kit employs a two-site sandwich ELISA to quantitate NADK in samples. An antibody specific for NADK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNADK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NADK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NADK bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human NAD kinase (NADK) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.