Dock4 is part of a large class of proteins (GEFs) which contibrute to cellular signalling events by activating small G proteins. In their resting state G proteins are bound to Guanosine diphosphate (GDP) and their activation requires the dissociation of GDP and binding of guanosine triphosphate (GTP). GEFs activate G proteins by promoting this nucleotide exchange.The domain arrangement of Dock4 is largely equivalent to that of Dock180 (the archetypal member of the DOCK family) and other DOCK-A/B family members (35% sequence identity with Dock180, 39% with Dock2 and 54% with Dock3). Dock4, however, contains a unique set of motifs at its proline-rich C-terminus which include a Src-binding site that is shared with CED-5, the C. elegans ortholog of mammalian DOCK proteins.

Human Dedicator of cytokinesis protein 4 (DOCK4) ELISA Kit employs a two-site sandwich ELISA to quantitate DOCK4 in samples. An antibody specific for DOCK4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDOCK4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DOCK4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DOCK4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Dedicator of cytokinesis protein 4 (DOCK4) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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