DUSP9 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product shows selectivity for members of the ERK family of MAP kinases, is expressed only in placenta, kidney, and fetal liver, and is localized to the cytoplasm and nucleus.

Human Dual specificity protein phosphatase 9 (DUSP9) ELISA Kit employs a two-site sandwich ELISA to quantitate DUSP9 in samples. An antibody specific for DUSP9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDUSP9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DUSP9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DUSP9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Dual specificity protein phosphatase 9 (DUSP9) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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