Members of the perilipin family, such as PLIN4, coat intracellular lipid storage droplets The predicted 1,348-amino acid protein is homologous to the mouse plasma membrane-associated protein S3-12. RT-PCR ELISA detected ubiquitous expression of PLIN4, with highest expression in skeletal muscle, followed by heart and liver, and lowest expression in adult whole brain. Using immunofluorescence microscopy, Wolins et al. (2003) demonstrated that supplementation of mouse adipocytes with fatty acids resulted in a shift from diffuse S3-12 cytoplasmic expression to S3-12 coating of lipid droplets on the cell periphery. Over time, Plin1 coexpression was observed. Formation of S3-12-coated droplets required glucose and fatty acids that could be incorporated into triacylglycerol and was dependent on insulin.

Human Perilipin-4 (KIAA1881) ELISA Kit employs a two-site sandwich ELISA to quantitate KIAA1881 in samples. An antibody specific for KIAA1881 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKIAA1881 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KIAA1881 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIAA1881 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Perilipin-4 (KIAA1881) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-perilipin-4-kiaa1881-elisa-kit-kte61921/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.