KLb encodes a 1,043-amino acid protein that shares 41.2% identity with mouse Kl. Like Kl, Klb contains an N-terminal signal sequence, followed by 2 tandemly repeated glycosidase-like domains, a C-terminal transmembrane domain, and a short cytoplasmic tail. Both Kl and Klb lack 2 key glutamates in the predicted active centers of the glycosidase-like domains.Northern blot analysis of adult mouse tissues showed that Klb was predominantly expressed in liver and pancreas, with lower levels in skin and weak expression in stomach, skeletal muscle, small intestine, and lung. In mouse embryos, Klb expression became detectable at day 11 and increased in intensity as development progressed. In situ hybridization of mouse embryos revealed strong Klb expression in yolk sac, gut, brown and white adipose tissue, liver, and pancreas.

Human Beta-klotho (KLB) ELISA Kit employs a two-site sandwich ELISA to quantitate KLB in samples. An antibody specific for KLB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Beta-klotho (KLB) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.