Lacritin is a 12.3 kDa glycoprotein encoded in humans by the LACRT gene. Lacritin is a secreted protein found in tears and saliva. Lacritin also promotes tear secretion and proliferation of some epithelial cells.Lacritin is thus a prosecretory mitogen. Most lacritin is produced by the lacrimal gland. Some lacritin is produced by the meibomian gland, and also by epithelial cells of the conjunctiva and cornea. Together these epithelia comprise much of the lacrimal functional unit (LFU). Dry eye is the most common disease of the LFU. Preliminary studies with small trials suggest that lacritin may be differentially downregulated in dry eye. Topical lacritin promotes tearing in rabbit preclinical studies. Lacritin cell targeting is dependent on the cell surface heparan sulfate proteoglycan syndecan-1 (SDC1).

Human Extracellular glycoprotein lacritin (LACRT) ELISA Kit employs a two-site sandwich ELISA to quantitate LACRT in samples. An antibody specific for LACRT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLACRT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LACRT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LACRT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Extracellular glycoprotein lacritin (LACRT) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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