LASP1 encodes a member of a LIM protein subfamily which is characterized by a LIM motif and a domain of Src homology region 3. This protein functions as an actin-binding protein and possibly in cytoskeletal organization.In breast cancer cell lines, overexpression of the 4 kb MLN50 mRNA was correlated with amplification of the gene and with amplification and overexpression of ERBB2, which maps to the same region. The authors suggested that the 2 genes belong to the same amplicon. Amplification of chromosomal region 17q11-q21 is one of the most common events occurring in human breast cancers.Northern blot analysis revealed that LASP1 mRNA was expressed at a basal level in all normal tissues examined and overexpressed in 8% of primary breast cancers.

Human LIM and SH3 domain protein 1 (LASP1) ELISA Kit employs a two-site sandwich ELISA to quantitate LASP1 in samples. An antibody specific for LASP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLASP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LASP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LASP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human LIM and SH3 domain protein 1 (LASP1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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