Linker of Activated T cells also known as LAT is a protein which is encoded by the LAT gene. Alternative splicing results in multiple transcript variants encoding different isoforms.The protein encoded by this gene is phosphorylated by ZAP70/SYK protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts (also known as glycosphingolipid-enriched microdomains or GEMs) and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement.

Human Linker for activation of T cell (LAT) ELISA Kit employs a two-site sandwich ELISA to quantitate LAT in samples. An antibody specific for LAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Linker for activation of T cell (LAT) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.