Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHC is testis-specific and belongs to the lactate dehydrogenase family. Two transcript variants have been detected which differ in the 5 untranslated region.
Human L-lactate dehydrogenase C chain (LDHC) ELISA Kit employs a two-site sandwich ELISA to quantitate LDHC in samples. An antibody specific for LDHC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLDHC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LDHC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LDHC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human L-lactate dehydrogenase C chain (LDHC) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-l-lactate-dehydrogenase-c-chain-ldhc-elisa-kit-kte61840/
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