Galectins constitute a family of proteins that bind to β-galactoside residues and have diverse physiological functions. Report on the identification of a galectin-like molecule, galectin-12, in a human adipose tissue cDNA library. The protein contained two potential carbohydrate-recognition domains with the second carbohydrate-recognition domain being less conserved compared with other galectins.Galectin-12 mRNA was predominantly expressed in adipose tissue of human and mouse and in differentiated 3T3-L1 adipocytes. Caloric restriction and treatment of obese animals with troglitazone increased galectin-12 mRNA levels and decreased the average size of the cells in adipose tissue.

Human Galectin-12 (LGALS12) ELISA Kit employs a two-site sandwich ELISA to quantitate LGALS12 in samples. An antibody specific for LGALS12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLGALS12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGALS12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGALS12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Galectin-12 (LGALS12) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-galectin-12-lgals12-elisa-kit-kte61824/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.