Lmf1 RNA was expressed at higher levels in tissues that express little or no lipase activity such as testis and pancreas, suggesting that Lmf1 may have additional functions in these tissues. The Lmf1 protein was predicted to contain 5 transmembrane domains and an evolutionarily conserved domain comprising most of the C-terminal end. Colocalization with calnexin demonstrated that the Lmf1 protein is localized to the endoplasmic reticulum (ER).The resulting LMF1 truncation, tyr439 to stop (Y439X), removed 127 residues from the C terminus compared with 212 residues eliminated by the cld mutation in the mouse. The Y439X subject showed markedly high plasma triglyceride and had undergone repeated episodes of pancreatitis.

Human Lipase maturation factor 1 (LMF1) ELISA Kit employs a two-site sandwich ELISA to quantitate LMF1 in samples. An antibody specific for LMF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLMF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LMF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LMF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Lipase maturation factor 1 (LMF1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.