MACC1 is a key regulator of the hepatocyte growth factor (HGF)-HGF receptor (HGFR, or MET) pathway, which is involved in cellular growth, epithelial-mesenchymal transition, angiogenesis, cell motility, invasiveness, and metastasis. Expression of MACC1 in colon cancer specimens is an independent prognostic indicator for metastasis formation and metastasis-free survival.The deduced 852-amino acid has an SH3 domain, a proline-rich motif for binding SH3 domain-containing proteins, additional binding motifs for SH2 and SH3 domain-containing proteins, EPS15 homology domains, clathrin and retinoblastoma protein (RB1) interaction domains, and putative tyrosine phosphorylation sites. It shares about 44% identity with SH3BP4, and putative orthologs were identified in chicken, mouse, rat, dog, and chimp.

Human Metastasis-associated in colon cancer protein 1 (MACC1) ELISA Kit employs a two-site sandwich ELISA to quantitate MACC1 in samples. An antibody specific for MACC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMACC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MACC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MACC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Metastasis-associated in colon cancer protein 1 (MACC1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.