MAD2L1BPwas identified as a binding protein of the MAD2 mitotic arrest deficient-like 1 (MAD2/MAD2L1). This protein may interact with the spindle checkpoint and coordinate cell cycle events in late mitosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. Upon the completion of spindle attachment, formation of the CMT2-MAD2 complex coincides with dissociation of the p55CDC-MAD2 complex. Overexpression of CMT2 in cells arrested by the spindle checkpoint causes premature destruction of securin and allows exit from mitosis without chromosome segregation. Depletion of CMT2 induces cell death following a transient delay in the onset of anaphase. These results indicate that CMT3 interacts with the spindle checkpoint and coordinates cell cycle events in late mitosis.

Human MAD2L1-binding protein (MAD2L1BP) ELISA Kit employs a two-site sandwich ELISA to quantitate MAD2L1BP in samples. An antibody specific for MAD2L1BP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAD2L1BP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAD2L1BP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAD2L1BP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human MAD2L1-binding protein (MAD2L1BP) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.