Microtubule-associated protein 2 is a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis.MAP2 serves to stabilize microtubules (MT) growth by crosslinking MT with intermediate filaments and other MT. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dendrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isforms have been described.

Human Microtubule-associated protein 2 (MAP-2) ELISA Kit employs a two-site sandwich ELISA to quantitate MAP2 in samples. An antibody specific for MAP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Microtubule-associated protein 2 (MAP-2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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