Mitogen-activated protein kinase 14 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response.

Human Mitogen-activated protein kinase 14 (MAPK14) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPK14 in samples. An antibody specific for MAPK14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPK14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPK14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPK14 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Mitogen-activated protein kinase 14 (MAPK14) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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