The deduced 1,265-amino acid protein shares significant similarity with mouse Mast205. RT-PCR ELISA detected variable MAST2 expression in all tissues examined. The deduced 1,734-amino acid protein has a calculated molecular mass of 190.5 kD. It had an apparent molecular mass of 205 kD by SDS-PAGE. Mast205 contains a central serine/threonine kinase catalytic domain, which includes an ATP-binding consensus sequence, and several potential phosphorylation sites. Northern blot analysis of mouse tissues detected Mast205 expression only in testis. Mast205 expression increased during postnatal testicular development coincident with meiosis/spermiogenesis. Immunofluorescence microscopy showed Mast205 associated with mouse spermatid manchette.

Human Microtubule-associated serine/threonine-protein kinase 2 (MAST2) ELISA Kit employs a two-site sandwich ELISA to quantitate MAST2 in samples. An antibody specific for MAST2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAST2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAST2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAST2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Microtubule-associated serine/threonine-protein kinase 2 (MAST2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.