The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. Mediator of RNA polymerase II transcription subunit 14 encoded by MED14 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Mediator of RNA polymerase II transcription subunit 14 contains a bipartite nuclear localization signal. MED14 is known to escape chromosome X-inactivation.

Human Mediator of RNA polymerase II transcription subunit 14 (MED14) ELISA Kit employs a two-site sandwich ELISA to quantitate MED14 in samples. An antibody specific for MED14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED14 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Mediator of RNA polymerase II transcription subunit 14 (MED14) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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