The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by MED17 is a subunit of the CRSP (cofactor required f Mediator of RNA polymerase II transcription subunit 17 is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

Human Mediator of RNA polymerase II transcription subunit 17 (MED17) ELISA Kit employs a two-site sandwich ELISA to quantitate MED17 in samples. An antibody specific for MED17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED17 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED17 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Mediator of RNA polymerase II transcription subunit 17 (MED17) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.