MED20 encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder. Alternatively spliced transcript variants have been identified.

Human Mediator of RNA polymerase II transcription subunit 20 (MED20) ELISA Kit employs a two-site sandwich ELISA to quantitate MED20 in samples. An antibody specific for MED20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED20 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED20 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Mediator of RNA polymerase II transcription subunit 20 (MED20) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-mediator-of-rna-polymerase-ii-transcription-subunit-20-med20-elisa-kit-kte61663/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.