Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A encoded by MGAT5 belongs to the glycosyltransferase family. It catalyzes the addition of beta-1,6-N-acetylglucosamine to the alpha-linked mannose of biantennary N-linked oligosaccharides present on the newly synthesized glycoproteins. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. Alterations of the oligosaccharides on cell surface glycoproteins cause significant changes in the adhesive or migratory behavior of a cell. Increase in the activity of this enzyme has been correlated with the progression of invasive malignancies. 

Human Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (MGAT5) ELISA Kit employs a two-site sandwich ELISA to quantitate MGAT5 in samples. An antibody specific for MGAT5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMGAT5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MGAT5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MGAT5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (MGAT5) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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