IgM is a particular isotype of Antibody. When IgM is expressed as a membrane protein it is termed the "B-cell Receptor". In the membrane-bound form, binding of antigen to IgM can induces a variety of signal transduction cascades in the B-cell which indicate that the B-cell Receptor has encountered antigen. Consequently, expression of membrane IgM as B-cell receptor is the primary format in which B-cells express antibody during B-cell Development and the initial stages of the Humoral Immune Response.

Human Membrane IgM (mIgM) ELISA Kit employs a two-site sandwich ELISA to quantitate mIgM in samples. An antibody specific for mIgM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anymIgM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for mIgM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of mIgM bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Membrane IgM (mIgM) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-membrane-igm-migm-elisa-kit-kte61614/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.