Mucin3 encoded by MUC3 is a membrane-bound mucin that provides protection to gut epithelial cells. Mucin3 contains about 60 tandem repeats, with each repeat being around 60 aa. N-glycosylation enables the Mucin3 to localize on the cell surface, while the C-terminus interacts with the scaffold protein PDZ domain containing 1 (PDZK1). Two transcript variants, one protein-coding and the other non-protein coding, have been found for MUC3.

Human Mucin3 (MUC3) ELISA Kit employs a two-site sandwich ELISA to quantitate MUC17 in samples. An antibody specific for MUC17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMUC17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC17 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC17 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Mucin3 (MUC3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.