Myosin-IIIa encoded by MYO3A belongs to the myosin superfamily. Myosins are actin-dependent motor proteins and are categorized into conventional myosins (class II) and unconventional myosins (classes I and III through XV) based on their variable C-terminal cargo-binding domains. Class III myosins, such as this one, have a kinase domain N-terminal to the conserved N-terminal motor domains and are expressed in photoreceptors. Myosin-IIIa encoded by MYO3A plays an important role in hearing in humans. Three different recessive, loss of function mutations in the encoded protein have been shown to cause nonsyndromic progressive hearing loss. Expression of MYO3A is highly restricted, with the strongest expression in retina and cochlea.

Human Myosin-IIIa (MYO3A) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO3A in samples. An antibody specific for MYO3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO3A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Myosin-IIIa (MYO3A) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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