NAF1 (Nuclear Assembly Factor 1 Ribonucleoprotein) is a Protein Coding gene. GO annotations related to this gene include poly(A) RNA binding and RNA binding. RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disappears during maturation of the complex and is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. Probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4.

Human H/ACA ribonucleoprotein complex non-core subunit NAF1 (NAF1) ELISA Kit employs a two-site sandwich ELISA to quantitate NAF1 in samples. An antibody specific for NAF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNAF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human H/ACA ribonucleoprotein complex non-core subunit NAF1 (NAF1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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