Autophagy cargo receptor encoded by NBR1 was originally identified as an ovarian tumor antigen monitored in ovarian cancer. Autophagy cargo receptor contains a B-box/coiled-coil motif, which is present in many genes with transformation potential. It functions as a specific autophagy receptor for the selective autophagic degradation of peroxisomes by forming intracellular inclusions with ubiquitylated autophagic substrates. NBR1 is located on a region of chromosome 17q21.1 that is in close proximity to the BRCA1 tumor suppressor gene. Alternative splicing of this gene results in multiple transcript variants. 

Human Next to BRCA1 gene 1 protein (NBR1) ELISA Kit employs a two-site sandwich ELISA to quantitate NBR1 in samples. An antibody specific for NBR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNBR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NBR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NBR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Next to BRCA1 gene 1 protein (NBR1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-next-to-brca1-gene-1-protein-nbr1-elisa-kit-kte61353/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.