Nuclear receptor coactivator 3 encoded by NCOA3 is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. Nuclear receptor coactivator 3 has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. Nuclear receptor coactivator 3 is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for NCOA3. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.

Human Nuclear receptor coactivator 3 (NCOA3) ELISA Kit employs a two-site sandwich ELISA to quantitate NCOA3 in samples. An antibody specific for NCOA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNCOA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NCOA3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NCOA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Nuclear receptor coactivator 3 (NCOA3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-nuclear-receptor-coactivator-3-ncoa3-elisa-kit-kte61343/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.