NIN encodes one of the proteins important for centrosomal function. Ninein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported.

Human Ninein (NIN) ELISA Kit employs a two-site sandwich ELISA to quantitate NIN in samples. An antibody specific for NIN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNIN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NIN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NIN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Ninein (NIN) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-ninein-nin-elisa-kit-kte61335/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.