Peptidyl-glycine alpha-amidating monooxygenase is a multifunctional protein. It has two enzymatically active domains with catalytic activities - peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL).These catalytic domains work sequentially to catalyze neuroendocrine peptides to active alpha-amidated products. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene but some of their full length sequences are not yet known.

Human Peptidyl-glycine alpha-amidating monooxygenase (PAM) ELISA Kit employs a two-site sandwich ELISA to quantitate PAM in samples. An antibody specific for PAM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAM bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Peptidyl-glycine alpha-amidating monooxygenase (PAM) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-peptidyl-glycine-alpha-amidating-monooxygenase-pam-elisa-kit-kte61310/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.