The LIM domain of PDLIM1 interacts with the C-terminal EF-hand region of alpha-actinin-2 (ACTN2). Immunoprecipitation, Western blot analysis, and immunofluorescence microscopy demonstrated that the 36-kD PDLIM1 protein colocalizes with ACTN2 in the Z discs of myocardial sarcomeres, particularly at intercalated discs, and with vinculin (VCL), which is localized in the fascia adherens of intercalated discs.Sequence analysis predicted that the 329-amino acid PDLIM1 protein, which is 88% homologous to the rat protein, shares 51 to 61% identity with the PDZ domains of ENIGMA, ALP, ENH, and RIL. SDS-PAGE analysis showed that recombinant PDLIM1 was expressed as a 36-kD protein.

Human PDZ and LIM domain protein 1 (PDLIM1) ELISA Kit employs a two-site sandwich ELISA to quantitate PDLIM1 in samples. An antibody specific for PDLIM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDLIM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDLIM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDLIM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human PDZ and LIM domain protein 1 (PDLIM1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.