The PGM2 protein shares about 20% identity with mammalian PGM1. Sequence analysis suggested that PGM2 is a cytosolic protein. Quantitative RT-PCR of mouse tissues detected the highest expression levels of PGM2 in lung, spleen, and thymus. PGM2 acted more than 10-fold better as a phosphopentomutasethan as a phosphoglucomutase. PGM2 may play a role in congenital immunodeficiencies. phosphopentomutase uses ribose 1-phosphate and deoxyribose 1-phosphate, which are formed by purine nucleoside phosphorylase and uridine phosphorylase, and that the absence of phosphopentomutase should result in the accumulation of ribose 1-phosphate and deoxyribose 1-phosphate and therefore in a functional block of purine nucleoside phosphorylase.

Human Phosphoglucomutase-2 (PGM2) ELISA Kit employs a two-site sandwich ELISA to quantitate PGM2 in samples. An antibody specific for PGM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Phosphoglucomutase-2 (PGM2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.