Elafin is an elastase-specific inhibitor, which contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Chang et al. and Schalkwijk et al. described a low molecular weight elastase inhibitor derived from psoriatic skin and termed SKALP for 'skin-derived antileukoproteinase.' They demonstrated that this inhibitor can be found in the epidermis of several inflammatory skin diseases, but not in normal human epidermis. Wiedow et al. referred to this molecule as elafin, and Sallenave and Ryle referred to it as elastase-specific inhibitor. It is found in the urine of psoriatic patients, and immunohistochemical studies showed that SKALP/elafin is found in the suprabasal differentiated keratinocytes of psoriatic epidermis. Molhuizen et al. isolated a precursor of SKALP/elafin from cultured keratinocytes and cloned its cDNA.

Human Elafin (PI3) ELISA Kit employs a two-site sandwich ELISA to quantitate PI3 in samples. An antibody specific for PI3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPI3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PI3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PI3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Elafin (PI3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.