PI4Ka catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. The mammalian PI 4-kinases have been classified into two types, II and III, based on their molecular mass, and modulation by detergent and adenosine. Two transcript variants encoding different isoforms have been described for this gene.The predicted 854-amino acid protein has a calculated molecular mass of 97 kD. It contains an ankyrin repeat, a lipid kinase unique domain, a pleckstrin-homology domain, and a lipid kinase catalytic domain. PI4K-alpha has high amino acid sequence similarity to the yeast PI 4-kinases STT4 and PIK1; it has less amino acid sequence similarity to the catalytic domains of mammalian and yeast PI 3-kinases and to the yeast TOR family of proteins.

Human Phosphatidylinositol 4-kinase alpha (PI4KA) ELISA Kit employs a two-site sandwich ELISA to quantitate PI4KA in samples. An antibody specific for PI4KA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPI4KA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PI4KA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PI4KA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Phosphatidylinositol 4-kinase alpha (PI4KA) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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