PIBF encodes a predicted hydrophilic 757-amino acid alpha-helical protein with an N-terminal signal sequence, a nuclear localization signal, a leucine zipper motif, a basic zipper sequence, a PEST sequence, an endoplasmic reticulum membrane retention signal, and several potential N-glycosylation and phosphorylation sites. Immunoblot analysis, immunocytochemistry, and fluorescence microscopy of insulin-treated mammary carcinoma cells detected expression of a 34-kD secreted protein in cytoplasmic granules. In untreated cells, nucleus-associated expression of the full-length 90-kD protein was detected.PIBF1 contains 758 amino acids. multiple PIBF1 transcripts expressed at highest levels in testis. Moderate expression was detected in spleen, thymus, prostate, ovary, small intestine, and colon.

Human Progesterone induced blocking factor (PIBF) ELISA Kit employs a two-site sandwich ELISA to quantitate PIBF1 in samples. An antibody specific for PIBF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIBF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIBF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIBF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Progesterone induced blocking factor (PIBF) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.