The PR domain is a protein-protein interaction module of about 100 amino acids. PR domain-containing proteins, such as PRDM9, are often involved in transcriptional regulation.The deduced 862-amino acid Meisetz protein contains a PR/SET domain in its N-terminal portion and a zinc finger domain containing multiple C2H2-type zinc fingers in its C-terminal portion. Hayashi et al. (2005) also cloned 2 Meisetz splice variants that encode shorter isoforms lacking the zinc finger domain. The PR/SET domain of Meisetz is highly conserved in human PRDM9. In situ hybridization, RT-PCR, and Northern blot analysis showed that mouse Meisetz transcripts were only expressed in germ cells entering meiotic prophase in female fetal gonads and in postnatal testis.

Human Histone-lysine N-methyltransferase PRDM9 (PRDM9) ELISA Kit employs a two-site sandwich ELISA to quantitate PRDM9 in samples. An antibody specific for PRDM9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRDM9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDM9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDM9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Histone-lysine N-methyltransferase PRDM9 (PRDM9) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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