Coagulated Blood DNA Mini-extraction Kit(Spin-column)
Principle
The kit applies special columns to separate blood blots according to the characteristics of whole blood to rapidly isolate DNA. First erythrocyte lysis buffer removes DNA-free erythrocyte. Nuclear lysis buffer splits leukocyte and DNA is released. Then protein precipitation buffer precipitates and removes protein selectively. Last the purified DNA is precipitated by isopropanol, and then DNA is dissolved in DNA dissolving buffer.
Features
1.Not contain phenol and other harmful compounds not required.
2.Simple and rapid. One preparation can be completed in 1 hour.
3.Stable result and high yield (the typical yield of 10ml whole blood about 150-500μg), high purity, the value of OD260/OD280 achieving 1.7-1.9. The Length of the genomic DNA is 50kb-150kb ,which can be applied to PCR,Southern-blot and digestions directly.
bio-equip.cn
BIOTEKE is a leading biotechnology company in China since 2005,
Our products nucleic acid extraction/purification as well as related essential lab instruments.
Please check our website here: http://english.bioteke.cn/
Our main product portfolio includes but not limits the following items,
Reagents/kits for molecular biological research uses,
DNA/RNA/plasmid extraction kits, PCR/RT-PCR/qPCR products, DNA/RNA stains/markers.
Automated Nucleic Acid Extraction systems, together with it's magnetic beeds kits;
For extracting DNA/RNA from various samples(cfDNA, blood, tissue/cells, plants, plasmid, etc) ,
32 samples or 96 samples in one time in 30min,
The cost for one samples is less than 4USD for our clients.
Micro Spectrophotometer ND5000,
0.5-2uL sample is needed, 4s for 1 test, to test the concentration of DNA/RNA/proteins.
Storage tubes for blood RNA, cfDNA, Urine DNA, swab/saliva DNA, stool DNA samples.
samples can be transported for more than one week, or for long term storage in low temprature.
widely used in clinical sample collections.Trial samples are available.
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